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Sheep Polyclonal anti Human EGF

Size Price Quantity
100.0 ug $290.00
Catalog Number hAP-5150
Sheep Polyclonal anti Human EGF
Size 50µg
Immunogen E. coli-derived recombinant human EGF
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified human EGF protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at -70 C.
Specificity Detects human EGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross reactivity with recombinant rat EGF is observed, 10% cross reactivity with recombinant mouse EGF, and less than 1% cross reactivity with recombinant human (rh) AR, rhBTC, rhHB-EGF, rhHRG-alpha, or rhHRG1-beta1 is observed.
Reconstitution Reconstitute the antibody with 100 µl sterile PBS and the final concentration is 500µg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
A.WB-(1 to 1000-2000)
B.IHC(Paraffin/Frozen)-(1 to 50-100)