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Sheep Polyclonal anti Human CCL4 (MIP-1 beta)


Item#:
hAP-5075
Size Price Quantity
100.0 ug $290.00
Catalog Number hAP-5075
Sheep Polyclonal anti Human CCL4 (MIP-1 beta)
Size 50µg
Immunogen S. Frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1beta
Clone Polyclonal AB
Antibody Isotype IgG
Preparation This antibody was produced from a sheep immunized with purified Human Ccl4/Mip-1beta Protein.
Formulation and Storage The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at -70 C.
Specificity Detects human CCL4/MIP-1beta in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) MIP-1alpha is observed, approximately 20% cross-reactivity with recombinant human (rh) MIP-1alpha and recombinant cotton rat MIP-1alpha is observed, and less than 1% cross-reactivity with rhMCP-2, rmMIP-1beta, rmMCP-2, and recombinant viral MIP-1beta is observed.
Reconstitution Reconstitute the antibody with 100 µl sterile PBS and the final concentration is 500µg/ml. Reconstituted antibody can also be aliquoted and stored frozen at < -20 C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 1000-2000)
B.IHC(Paraffin/Frozen)-(1 to 50-100)
C.ICC-(1 to 100-200)
D.N/B-Yes

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