Angio-Proteomie is providing custom based service for Isolation and culturing of primary endothelial cells from variable tissues from common laboratory animals (including but not limited to mice, rats, rabbits).
In generals, customers specify the species and tissues/organs of their interests or provide fresh tissues of their choices, and Angio-Proteomie will prepare primary endothelial cells from the tissues of their interests.
Brief answers to common questions :
Turnaround time: 2-3 weeks
Methods used for endothelial isolation: PECAM-1 based cell purification after tissue dissociation with protein enzymes
Cell Validation: IF, FC or WB Assays for common EC markers, VE-Cadherin, Von Willebrand (vWF), VEGFR2, or Tie2.
Passage number of cells offered: P2/3, dependent on numbers of cell purification processes.
The numbers that the cells can be passaged down the line: between 4-10passages at 1:2 splitting ratio.
General formats of cell products: 2 x T25 Flasks of confluent cells or 2 frozen vials from the 2 X T25 Flask of cells (other formats can be arranged per customer’s needs)
Species: All small lab animals (including but not limited to mice, rats, and rabbits, both wild type and transgenic animals) and we are open to offer services using for any other species that customers need.
Cost: Flat rate of $750 for endothelial cells from one tissue or organ. We are committed to offer the most cost effective services in the filed.
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