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Rat Monoclonal anti-mouse Cathepsin A


Item#:
mAP-0160
Size Price Quantity
50.0 ug $245.00
100.0 ug $320.00
Catalogue Number mAP-0160
Rat Monoclonal anti-mouse Cathepsin A
Size 100ug
Immumogen Mouse recombinant protein of Cathepsin A
Clone RM0160-4A12
Antibody Isotype Rat IgG2
Preparation This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with mouse recombinant protein of Cathepsin-A).
Formulation and Storage The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from the date of receipt when stored at -70C
Specificity This antibody detects mouse Cathepsin-A in direct ELISAs and immunohistochemistry experiments. In western blots, it recognizes the single chain (55 kDa) form of rmCathepsin A, which can be processed into heavy (32 kDa) and light (20 kDa) chains. It recognizes the heavy chain, but not the light chain
Reconstitution Reconstitute the antibody with 200 ul sterile PBS (final concentration 500ug/ml). Reconstituted antibody can be aliquoted and stored frozen at < -20 for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Applications
A.WB-(1 to 500-1000)
B. IHC(Frozen)-(1 to 100-400)
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D.
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