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Vascular Endothelial Growth Factor (121 a.a.) Human Recombinant


Item#:
rAP-2500
Size Price Quantity
2.0 ug $50.00
10.0 ug $130.00
1000.0 ug $3,500.00
Product Name Vascular Endothelial Growth Factor (121 a.a.) Human Recombinant
Synonyms Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Description Vascular Endothelial Growth Factor-121 Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 28423 Dalton. VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.The VEGF-121 is purified by proprietary chromatographic techniques.
Uniprot Accesion Number P15692
Amino Acid SequenceAPMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized Vascular Endothelial Growth Factor 121 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-121 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity The protein was lyophilized from a concentrated (1mg/ml) solution with no additives. Greater than 98.0% as determined by
Application
Solubility It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor -121 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2-0.4 ng/ml corresponding to a specific activity of 2.5-5MU/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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