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Tumor Necrosis Factor-Alpha Rabbit Recombinant


Item#:
rAP-0752
Size Price Quantity
5.0 ug $50.00
20.0 ug $130.00
1000.0 ug $2,700.00
Product Name Tumor Necrosis Factor-Alpha Rabbit Recombinant
Synonyms Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2. 
Description Tumor Necrosis Factor-a Rabbit Recombinant consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. TNF-alpha was produced in E.Coli is a non-glycosylated, polypeptide chain having a molecular mass of 17.4 kDa for the individual subunit. The TNF-alpha is purified by standard chromatographic techniques.
Uniprot Accesion Number P04924
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ala-Ser-Arg.
Source Escherichia Coli.
Physical Appearance and Stability Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity TNF-alpha Rabbit was lyophilized after extensive dialysis against 20mM PB, pH7.4, 300mM NaCl. Greater than 95% as determined by
Application
Solubility It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 30,000,000 IU/mg.
Shipping Format and Condition Lyophilized powder at room temperature.
Usage Statement Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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