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name of test_01

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200.0 ug $200.00
Title Goat anti Human ABC antibody
CD1B Human Recombinant
CD1B protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol and 1mM DTT.
A)Pre-coating of T25 flasks
B)Rinse the cells in T25 flask with 5ml HBSS (Room Temperature, RT) twice.
C)Add 2ml of Trypsin/EDTA (RT) (cAP-23) into one T25 flask (make sure the whole surface of the T25 flask is covered with Trypsin/EDTA), and gently dispose the excessive Trypsin/EDTA solution within 20 seconds with aspiration.
D)Leave the T25 flask with the cells at RT for 1 minute (the cells usually will detach from the surface within 1-2 minutes). You can monitor the cells under microscope and when most of cells become rounded up, hit the flask against the bench surface, and the cells will move on the surface of the flask when monitoring under microscope.
E)Add 5ml Trypsin Neutralization Buffer and spin the cells down with 800g for 5 minutes.
F)Re-suspend the cell pellet with 10 - 15ml of EGM full medium and the cell suspension is transferred directly into 2 or 3 pre-coated T25 flasks (5ml each, and the cells are sub-cultured at 1
G)Change medium every 2-3 days and cells usually become confluent within 7 days (when split at a 1
H)If you need prepare quiescent cells, when cells are almost confluent, replace EGM full medium with Endothelial Basal Medium (EBM, cAP-03) containing 0.5% FBS about 8-12 hours before your experiments.
1.Cytoplasmic VWF / Factor VIII; >95% positive by immunofluorescence
2.Cytoplasmic uptake of Di-I-Ac-LDL>95% positive by immunofluorescence
3.Cytoplasmic PECAM1>95% positive by immunofluorescence
4.HUVECs are negative for HIV-1, HBV, HCV, and mycoplas